Chapter title |
Mass Spectrometry Imaging (MSI) for Plant Metabolomics
|
---|---|
Chapter number | 17 |
Book title |
Plant Metabolomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7819-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7818-2, 978-1-4939-7819-9
|
Authors |
Berin A. Boughton, Dinaiz Thinagaran |
Abstract |
Mass spectrometry imaging (MSI) is a developing technique to measure the spatiotemporal distribution of many biomolecules in tissues. Over the preceding decade MSI has been adopted by plant biologists and applied in a broad range of areas including: primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids, and the developing field of spatial metabolomics. This methods chapter covers preparation of plant tissues for matrix-assisted laser desorption ionization (MALDI)-MSI, including sample embedding and freezing, sectioning, mounting, and matrix deposition using both sublimation and spray deposition prior to MSI analysis. |
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Spain | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
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Scientists | 1 | 17% |
Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
Geographical breakdown
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Unknown | 26 | 100% |
Demographic breakdown
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Student > Master | 6 | 23% |
Student > Ph. D. Student | 4 | 15% |
Other | 3 | 12% |
Professor | 1 | 4% |
Researcher | 1 | 4% |
Other | 2 | 8% |
Unknown | 9 | 35% |
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Biochemistry, Genetics and Molecular Biology | 3 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Medicine and Dentistry | 1 | 4% |
Other | 1 | 4% |
Unknown | 11 | 42% |