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Electroporation Protocols

Overview of attention for book
Cover of 'Electroporation Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments
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    Chapter 2 Electropermeabilization of the Cell Membrane
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    Chapter 3 The Impact of Non-electrical Factors on Electrical Gene Transfer.
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    Chapter 4 Electroporation formulation for cell therapy.
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    Chapter 5 Short-Fragment DNA-Mediated In Vivo DNA Electroporation Delivery
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    Chapter 6 Gold nanoparticle-enhanced electroporation for leukemia cell transfection.
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    Chapter 7 siRNA Delivery via Electropulsation: A Review of the Basic Processes
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    Chapter 8 Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips
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    Chapter 9 Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells
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    Chapter 10 Direct Imaging of siRNA Electrotransfer at the Single-Cell Level.
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    Chapter 11 Electroporation Protocols
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    Chapter 12 Substrate-Mediated, High-Efficiency siRNA Electroporation
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    Chapter 13 Intradermal Electroporation of RNA
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    Chapter 14 Delivery of DNA into the Central Nervous System via Electroporation
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    Chapter 15 DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes
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    Chapter 16 Delivery of Plasmid DNA into Dental Tissues of Developing Rat Teeth by Electroporation
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    Chapter 17 Electroporation-mediated gene delivery to the lungs.
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    Chapter 18 Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion
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    Chapter 19 In vivo electroporation-mediated gene delivery to the beating heart.
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    Chapter 20 Managing local swelling following intratumoral electro-chemo-gene therapy.
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    Chapter 21 Gene Electrotransfer in Clinical Trials
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    Chapter 22 Electrochemotherapy in Veterinary Oncology: From Rescue to First Line Therapy
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    Chapter 23 Low-Energy DC Current Ablation in a Mouse Tumor Model.
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    Chapter 24 Evolution of Electroporated DNA Vaccines
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    Chapter 25 Clinical Development of Intramuscular Electroporation: Providing a “Boost” for DNA Vaccines
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    Chapter 26 Electroporation-Mediated Administration of Candidate DNA Vaccines Against HIV-1
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    Chapter 27 Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates
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    Chapter 28 Intramuscular DNA Vaccination Protocols Mediated by Electric Fields
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    Chapter 29 DNA Electroporation of Multi-agent Vaccines Conferring Protection Against Select Agent Challenge: TriGrid Delivery System
  31. Altmetric Badge
    Chapter 30 Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies
Attention for Chapter 28: Intramuscular DNA Vaccination Protocols Mediated by Electric Fields
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  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

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Chapter title
Intramuscular DNA Vaccination Protocols Mediated by Electric Fields
Chapter number 28
Book title
Electroporation Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4614-9632-8_28
Pubmed ID
24510835
Book ISBNs
978-1-4614-9631-1, 978-1-4614-9632-8
Authors

Pieranna Chiarella, Emanuela Signori, Chiarella, Pieranna, Signori, Emanuela

Abstract

Vaccination is historically one of the most important methods for preventing infectious diseases in humans and animals. New insights in the biology of the immune system allow a more rational design of vaccines, and new vaccination strategies are emerging. DNA vaccines have been proposed as a promising approach for introducing foreign antigens into the host for inducing protective immunity against infectious and cancer diseases. Nevertheless, because of their poor immunogenicity, plasmid DNA vaccination strategies need further implementations. Recent data suggest electrotransfer as a useful tool to improve DNA-based vaccination protocols, being able to stimulate both the humoral and cellular immune responses. In preclinical trials, gene electrotransfer is successfully used in prime-boost combination protocols and its tolerability and safety has been demonstrated also in Phase I clinical trials. In this chapter, we report a short comment supporting electrotransfer as an effective strategy to improve DNA-based vaccination protocols and describe the vaccination procedures by plasmid DNA in combination with electrotransfer and hyaluronidase pretreatment in use in our laboratory.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 25%
Researcher 2 25%
Professor 1 13%
Other 1 13%
Student > Master 1 13%
Other 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Medicine and Dentistry 3 38%
Materials Science 1 13%
Engineering 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2014.
All research outputs
#15,301,167
of 22,756,196 outputs
Outputs from Methods in molecular biology
#5,313
of 13,089 outputs
Outputs of similar age
#190,021
of 305,260 outputs
Outputs of similar age from Methods in molecular biology
#200
of 597 outputs
Altmetric has tracked 22,756,196 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,260 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 597 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.

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