Chapter title |
Electroporation-mediated gene delivery to the lungs.
|
---|---|
Chapter number | 17 |
Book title |
Electroporation Protocols
|
Published in |
Methods in molecular biology, February 2014
|
DOI | 10.1007/978-1-4614-9632-8_17 |
Pubmed ID | |
Book ISBNs |
978-1-4614-9631-1, 978-1-4614-9632-8
|
Authors |
Young JL, Barravecchia MS, Dean DA, Jennifer L. Young, Michael S. Barravecchia, David A. Dean, Young, Jennifer L., Barravecchia, Michael S., Dean, David A. |
Abstract |
Electroporation is a safe, efficient, and inexpensive method to transfer naked plasmid DNA into various tissues. For electroporation-mediated gene transfer to the mouse lung, a plasmid solution is delivered to the lungs via the trachea. Immediately after plasmid delivery, eight square wave pulses are delivered by two pre-gelled electrodes placed on each side of the chest. An optimal field strength in mice is 200 V/cm, with a pulse duration of 10 ms each and a 1 s interval between pulses. High level gene expression can be achieved within 24 h in all cell types in the lung with very little inflammation and no apparent trauma. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 17% |
Student > Bachelor | 2 | 11% |
Student > Ph. D. Student | 2 | 11% |
Student > Doctoral Student | 1 | 6% |
Student > Master | 1 | 6% |
Other | 3 | 17% |
Unknown | 6 | 33% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 2 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Materials Science | 1 | 6% |
Other | 1 | 6% |
Unknown | 6 | 33% |