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Pseudomonas Methods and Protocols

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Cover of 'Pseudomonas Methods and Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Gene Transfer: Transduction
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    Chapter 2 Gene Transfer: Transformation/Electroporation
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    Chapter 3 Gene transfer: conjugation.
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    Chapter 4 Pseudomonas Bacteriophage Isolation and Production
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    Chapter 5 Genotyping Methods
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    Chapter 6 Drug Susceptibility Testing by Dilution Methods
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    Chapter 7 Plate-Based Assay for Swimming Motility in Pseudomonas aeruginosa
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    Chapter 8 Plate-Based Assay for Swarming Motility in Pseudomonas aeruginosa
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    Chapter 9 Motility Assay: Twitching Motility
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    Chapter 10 Qualitative and Quantitative Assays for Flagellum-Mediated Chemotaxis
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    Chapter 11 Microscopic Analysis: Morphotypes and Cellular Appendages
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    Chapter 12 Determination of Lipolytic Enzyme Activities
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    Chapter 13 Elastinolytic and Proteolytic Enzymes
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    Chapter 14 In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins
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    Chapter 15 Cell Fractionation
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    Chapter 16 Characterization of Molecular Interactions Using Isothermal Titration Calorimetry
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    Chapter 17 Proteomic analysis.
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    Chapter 18 Membrane Proteomics of Pseudomonas aeruginosa
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    Chapter 19 Construction of Pseudomonas aeruginosa Two-Hybrid Libraries for High-Throughput Assays
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    Chapter 20 Biosensors for Qualitative and Semiquantitative Analysis of Quorum Sensing Signal Molecules
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    Chapter 21 LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules
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    Chapter 22 LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP.
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    Chapter 23 Metabolic footprinting: extracellular metabolomic analysis.
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    Chapter 24 Pyoverdine and Pyochelin Measurements
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    Chapter 25 Measurement of Phenazines in Bacterial Cultures
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    Chapter 26 Extraction and Measurement of NAD(P) + and NAD(P)H
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    Chapter 27 Cyanide Measurements in Bacterial Culture and Sputum
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    Chapter 28 Monitoring iron uptake by siderophores.
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    Chapter 29 Exopolysaccharide quantification.
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    Chapter 30 Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids
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    Chapter 31 LPS Quantitation Procedures
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    Chapter 32 Monitoring lectin interactions with carbohydrates.
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    Chapter 33 Mining the Pseudomonas Genome
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    Chapter 34 Identification of Bacterial Small RNAs by RNA Sequencing.
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    Chapter 35 Gene Amplification and qRT-PCR
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    Chapter 36 The Standard European Vector Architecture (SEVA) Plasmid Toolkit
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    Chapter 37 Chromosomal integration of transcriptional fusions.
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    Chapter 38 A Method to Capture Large DNA Fragments from Genomic DNA
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    Chapter 39 Transposon mutagenesis.
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    Chapter 40 Site-Directed Mutagenesis and Gene Deletion Using Reverse Genetics
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    Chapter 41 Signature-Tagged Mutagenesis
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    Chapter 42 Construction of a Pseudomonas aeruginosa Genomic DNA Library
  44. Altmetric Badge
    Chapter 43 Pseudomonas Methods and Protocols
  45. Altmetric Badge
    Chapter 44 Promoter fusions with optical outputs in individual cells and in populations.
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    Chapter 45 Chromatin Immunoprecipitation for ChIP-chip and ChIP-seq.
  47. Altmetric Badge
    Chapter 46 Pseudomonas Methods and Protocols
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    Chapter 47 Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy.
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    Chapter 48 Biofilm formation in the 96-well microtiter plate.
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    Chapter 49 Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa
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    Chapter 50 Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis elegans
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    Chapter 51 Assessing Pseudomonas aeruginosa Virulence Using a Nonmammalian Host: Dictyostelium discoideum
  53. Altmetric Badge
    Chapter 52 Assessing Pseudomonas Virulence with Nonmammalian Host: Galleria mellonella
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    Chapter 53 Assessing Pseudomonas Virulence with the Nonmammalian Host Model: Arabidopsis thaliana
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    Chapter 54 Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells
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    Chapter 55 Assessing Pseudomonas Virulence with Nonmammalian Host: Zebrafish
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    Chapter 56 Assessing Pseudomonas Virulence with a Nonmammalian Host: Drosophila melanogaster.
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    Chapter 57 Assessing Pseudomonas Virulence Using Host Cells
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    Chapter 58 Assessing Pseudomonas aeruginosa Virulence and the Host Response Using Murine Models of Acute and Chronic Lung Infection
  60. Altmetric Badge
    Chapter 59 Assessing Pseudomonas Virulence Using Mammalian Models: Acute Infection Model
  61. Altmetric Badge
    Chapter 60 Burn Mouse Models
Attention for Chapter 45: Chromatin Immunoprecipitation for ChIP-chip and ChIP-seq.
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (58th percentile)
  • High Attention Score compared to outputs of the same age and source (86th percentile)

Mentioned by

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3 tweeters
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1 Google+ user

Citations

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Chapter title
Chromatin Immunoprecipitation for ChIP-chip and ChIP-seq.
Chapter number 45
Book title
Pseudomonas Methods and Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-0473-0_45
Pubmed ID
Book ISBNs
978-1-4939-0472-3, 978-1-4939-0473-0
Authors

Sebastian Schulz, Susanne Häussler

Abstract

Bacterial adaptation to given environmental conditions is largely achieved by complex gene regulatory processes. To address the question how and to what extend single transcriptional regulators modulate gene expression, chromatin immunoprecipitation (ChIP) coupled to DNA microarrays (ChIP-chip) or to next-generation sequencing (ChIP-seq) is one of the preferred methods. Both ChIP-chip and ChIP-seq can generate genome-wide maps of protein-DNA interactions and thus identify primary regulons of transcription factors. In combination with transcriptome analyses, the obtained data can be used to compile complex regulatory networks which in terms will advance our understanding of bacterial adaptation processes to specific environmental conditions.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 20%
Lecturer 1 20%
Student > Bachelor 1 20%
Student > Master 1 20%
Unspecified 1 20%
Other 0 0%
Readers by discipline Count As %
Unspecified 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%
Agricultural and Biological Sciences 1 20%
Medicine and Dentistry 1 20%
Design 1 20%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 April 2015.
All research outputs
#7,449,441
of 13,836,441 outputs
Outputs from Methods in molecular biology
#1,986
of 8,713 outputs
Outputs of similar age
#74,663
of 189,196 outputs
Outputs of similar age from Methods in molecular biology
#12
of 97 outputs
Altmetric has tracked 13,836,441 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,713 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 189,196 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 97 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.