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Pseudomonas Methods and Protocols

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Cover of 'Pseudomonas Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Gene Transfer: Transduction
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    Chapter 2 Gene Transfer: Transformation/Electroporation
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    Chapter 3 Gene transfer: conjugation.
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    Chapter 4 Pseudomonas Bacteriophage Isolation and Production
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    Chapter 5 Genotyping Methods
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    Chapter 6 Drug Susceptibility Testing by Dilution Methods
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    Chapter 7 Plate-Based Assay for Swimming Motility in Pseudomonas aeruginosa
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    Chapter 8 Plate-Based Assay for Swarming Motility in Pseudomonas aeruginosa
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    Chapter 9 Motility Assay: Twitching Motility
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    Chapter 10 Qualitative and Quantitative Assays for Flagellum-Mediated Chemotaxis
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    Chapter 11 Microscopic Analysis: Morphotypes and Cellular Appendages
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    Chapter 12 Determination of Lipolytic Enzyme Activities
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    Chapter 13 Elastinolytic and Proteolytic Enzymes
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    Chapter 14 In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins
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    Chapter 15 Cell Fractionation
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    Chapter 16 Characterization of Molecular Interactions Using Isothermal Titration Calorimetry
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    Chapter 17 Proteomic analysis.
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    Chapter 18 Membrane Proteomics of Pseudomonas aeruginosa
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    Chapter 19 Construction of Pseudomonas aeruginosa Two-Hybrid Libraries for High-Throughput Assays
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    Chapter 20 Biosensors for Qualitative and Semiquantitative Analysis of Quorum Sensing Signal Molecules
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    Chapter 21 LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules
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    Chapter 22 LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP.
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    Chapter 23 Metabolic footprinting: extracellular metabolomic analysis.
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    Chapter 24 Pyoverdine and Pyochelin Measurements
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    Chapter 25 Measurement of Phenazines in Bacterial Cultures
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    Chapter 26 Extraction and Measurement of NAD(P) + and NAD(P)H
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    Chapter 27 Cyanide Measurements in Bacterial Culture and Sputum
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    Chapter 28 Monitoring iron uptake by siderophores.
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    Chapter 29 Exopolysaccharide quantification.
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    Chapter 30 Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids
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    Chapter 31 LPS Quantitation Procedures
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    Chapter 32 Monitoring lectin interactions with carbohydrates.
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    Chapter 33 Mining the Pseudomonas Genome
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    Chapter 34 Identification of Bacterial Small RNAs by RNA Sequencing.
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    Chapter 35 Gene Amplification and qRT-PCR
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    Chapter 36 The Standard European Vector Architecture (SEVA) Plasmid Toolkit
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    Chapter 37 Chromosomal integration of transcriptional fusions.
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    Chapter 38 A Method to Capture Large DNA Fragments from Genomic DNA
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    Chapter 39 Transposon mutagenesis.
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    Chapter 40 Site-Directed Mutagenesis and Gene Deletion Using Reverse Genetics
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    Chapter 41 Signature-Tagged Mutagenesis
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    Chapter 42 Construction of a Pseudomonas aeruginosa Genomic DNA Library
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    Chapter 43 Pseudomonas Methods and Protocols
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    Chapter 44 Promoter fusions with optical outputs in individual cells and in populations.
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    Chapter 45 Chromatin Immunoprecipitation for ChIP-chip and ChIP-seq.
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    Chapter 46 Pseudomonas Methods and Protocols
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    Chapter 47 Methods for studying biofilm formation: flow cells and confocal laser scanning microscopy.
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    Chapter 48 Biofilm formation in the 96-well microtiter plate.
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    Chapter 49 Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa
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    Chapter 50 Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis elegans
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    Chapter 51 Assessing Pseudomonas aeruginosa Virulence Using a Nonmammalian Host: Dictyostelium discoideum
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    Chapter 52 Assessing Pseudomonas Virulence with Nonmammalian Host: Galleria mellonella
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    Chapter 53 Assessing Pseudomonas Virulence with the Nonmammalian Host Model: Arabidopsis thaliana
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    Chapter 54 Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells
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    Chapter 55 Assessing Pseudomonas Virulence with Nonmammalian Host: Zebrafish
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    Chapter 56 Assessing Pseudomonas Virulence with a Nonmammalian Host: Drosophila melanogaster.
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    Chapter 57 Assessing Pseudomonas Virulence Using Host Cells
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    Chapter 58 Assessing Pseudomonas aeruginosa Virulence and the Host Response Using Murine Models of Acute and Chronic Lung Infection
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    Chapter 59 Assessing Pseudomonas Virulence Using Mammalian Models: Acute Infection Model
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    Chapter 60 Burn Mouse Models
Attention for Chapter 35: Gene Amplification and qRT-PCR
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Chapter title
Gene Amplification and qRT-PCR
Chapter number 35
Book title
Pseudomonas Methods and Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-0473-0_35
Pubmed ID
Book ISBNs
978-1-4939-0472-3, 978-1-4939-0473-0
Authors

Cerith Jones, Alain Filloux

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 14%
Lecturer 1 14%
Professor 1 14%
Student > Ph. D. Student 1 14%
Student > Master 1 14%
Other 1 14%
Unknown 1 14%
Readers by discipline Count As %
Immunology and Microbiology 2 29%
Unspecified 1 14%
Agricultural and Biological Sciences 1 14%
Medicine and Dentistry 1 14%
Design 1 14%
Other 0 0%
Unknown 1 14%