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Homing Endonucleases

Overview of attention for book
Cover of 'Homing Endonucleases'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers
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    Chapter 2 Bioinformatic identification of homing endonucleases and their target sites.
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    Chapter 3 PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.
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    Chapter 4 Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein
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    Chapter 5 Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE
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    Chapter 6 PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis
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    Chapter 7 A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases.
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    Chapter 8 Rapid screening of endonuclease target site preference using a modified bacterial two-plasmid selection.
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    Chapter 9 A Yeast-Based Recombination Assay for Homing Endonuclease Activity
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    Chapter 10 Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile
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    Chapter 11 Quantifying the information content of homing endonuclease target sites by single base pair profiling.
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    Chapter 12 Homing endonuclease target site specificity defined by sequential enrichment and next-generation sequencing of highly complex target site libraries.
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    Chapter 13 Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display
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    Chapter 14 Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes.
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    Chapter 15 Bioinformatics identification of coevolving residues.
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    Chapter 16 Identification and Analysis of Genomic Homing Endonuclease Target Sites
  18. Altmetric Badge
    Chapter 17 Redesigning the Specificity of Protein-DNA Interactions with Rosetta.
Attention for Chapter 3: PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.
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  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

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Chapter title
PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes.
Chapter number 3
Book title
Homing Endonucleases
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-968-0_3
Pubmed ID
24510258
Book ISBNs
978-1-62703-967-3, 978-1-62703-968-0
Authors

Hafez M, Guha TK, Shen C, Sethuraman J, Hausner G, Mohamed Hafez, Tuhin Kumar Guha, Chen Shen, Jyothi Sethuraman, Georg Hausner, Hafez, Mohamed, Guha, Tuhin Kumar, Shen, Chen, Sethuraman, Jyothi, Hausner, Georg

Abstract

Fungal mitochondrial genomes act as "reservoirs" for homing endonucleases. These enzymes with their DNA site-specific cleavage activities are attractive tools for genome editing and gene therapy applications. Bioprospecting and characterization of naturally occurring homing endonucleases offers an alternative to synthesizing artificial endonucleases. Here, we describe methods for PCR-based screening of fungal mitochondrial rRNA genes for homing endonuclease encoding sequences, and we also provide protocols for the purification and biochemical characterization of putative native homing endonucleases.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 33%
Professor 1 17%
Researcher 1 17%
Unknown 2 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 67%
Unknown 2 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2014.
All research outputs
#15,299,491
of 22,753,345 outputs
Outputs from Methods in molecular biology
#5,313
of 13,089 outputs
Outputs of similar age
#192,936
of 313,185 outputs
Outputs of similar age from Methods in molecular biology
#209
of 658 outputs
Altmetric has tracked 22,753,345 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,185 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 658 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.

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