Chapter title |
Bioinformatic identification of homing endonucleases and their target sites.
|
---|---|
Chapter number | 2 |
Book title |
Homing Endonucleases
|
Published in |
Methods in molecular biology, February 2014
|
DOI | 10.1007/978-1-62703-968-0_2 |
Pubmed ID | |
Book ISBNs |
978-1-62703-967-3, 978-1-62703-968-0
|
Authors |
Privman E, Eyal Privman, Privman, Eyal |
Abstract |
Homing endonuclease genes (HEGs) are a large, phylogenetically diverse superfamily of enzymes with high specificity for especially long target sites. The public genomic sequence databases contain thousands of HEGs. This is a large and diverse arsenal of potential genome editing tools. To make use of this natural resource, one needs to identify candidate HEGs. Due to their special relationship with a host gene, it is also possible to predict their cognate target sequences. Here I describe the HomeBase algorithm that was developed to this end. A detailed description of the computational pipeline is provided with emphasis on technical and methodological caveats of the approach. |
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