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RNA Nanotechnology and Therapeutics

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Cover of 'RNA Nanotechnology and Therapeutics'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of methods in RNA nanotechnology: synthesis, purification, and characterization of RNA nanoparticles.
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    Chapter 2 Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly.
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    Chapter 3 Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.
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    Chapter 4 Silver nanoclusters for RNA nanotechnology: steps towards visualization and tracking of RNA nanoparticle assemblies.
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    Chapter 5 Large scale purification of RNA nanoparticles by preparative ultracentrifugation.
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    Chapter 6 HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single-Nucleotide Resolution
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    Chapter 7 Using RNA nanoparticles with thermostable motifs and fluorogenic modules for real-time detection of RNA folding and turnover in prokaryotic and eukaryotic cells.
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    Chapter 8 Fluorescence Labeling of Short RNA by Oxidation at the 3′-End
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    Chapter 9 Methods and assays for specific targeting and delivery of RNA nanoparticles to cancer metastases.
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    Chapter 10 Functional assays for specific targeting and delivery of RNA nanoparticles to brain tumor.
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    Chapter 11 Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens
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    Chapter 12 Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.
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    Chapter 13 A high-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells.
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    Chapter 14 Design, Assembly, and Evaluation of RNA-Protein Nanostructures.
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    Chapter 15 Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq
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    Chapter 16 Mapping Protein–RNA Interactions by RCAP, RNA-Cross-Linking and Peptide Fingerprinting
Attention for Chapter 16: Mapping Protein–RNA Interactions by RCAP, RNA-Cross-Linking and Peptide Fingerprinting
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Chapter title
Mapping Protein–RNA Interactions by RCAP, RNA-Cross-Linking and Peptide Fingerprinting
Chapter number 16
Book title
RNA Nanotechnology and Therapeutics
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2562-9_16
Pubmed ID
Book ISBNs
978-1-4939-2561-2, 978-1-4939-2562-9
Authors

Robert C. Vaughan, C. Cheng Kao, Vaughan, Robert C., Kao, C. Cheng

Abstract

RNA nanotechnology often feature protein RNA complexes. The interaction between proteins and large RNAs are difficult to study using traditional structure-based methods like NMR or X-ray crystallography. RCAP, an approach that uses reversible-cross-linking affinity purification method coupled with mass spectrometry, has been developed to map regions within proteins that contact RNA. This chapter details how RCAP is applied to map protein-RNA contacts within virions.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 33%
Professor 1 17%
Student > Ph. D. Student 1 17%
Researcher 1 17%
Student > Postgraduate 1 17%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 33%
Agricultural and Biological Sciences 2 33%
Chemistry 2 33%