| Chapter title |
Silver nanoclusters for RNA nanotechnology: steps towards visualization and tracking of RNA nanoparticle assemblies.
|
|---|---|
| Chapter number | 4 |
| Book title |
RNA Nanotechnology and Therapeutics
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2562-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2561-2, 978-1-4939-2562-9
|
| Authors |
Afonin, Kirill A, Schultz, Danielle, Jaeger, Luc, Gwinn, Elisabeth, Shapiro, Bruce A, Kirill A. Afonin, Danielle Schultz, Luc Jaeger, Elisabeth Gwinn, Bruce A. Shapiro, Afonin, Kirill A., Shapiro, Bruce A. |
| Abstract |
The growing interest in designing functionalized, RNA-based nanoparticles (NPs) for applications such as cancer therapeutics requires simple, efficient assembly assays. Common methods for tracking RNA assemblies such as native polyacrylamide gels and atomic force microscopy are often time-intensive and, therefore, undesirable. Here we describe a technique for rapid analysis of RNA NP assembly stages using the formation of fluorescent silver nanoclusters (Ag NCs). This method exploits the single-stranded specificity and sequence dependence of Ag NC formation to produce unique optical readouts for each stage of RNA NP assembly, obtained readily after synthesis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 7% |
| Unknown | 13 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 29% |
| Professor > Associate Professor | 2 | 14% |
| Student > Ph. D. Student | 2 | 14% |
| Professor | 1 | 7% |
| Other | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 14% |
| Chemistry | 2 | 14% |
| Physics and Astronomy | 2 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Mathematics | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 4 | 29% |