| Chapter title |
Measurement of Oxidative Burst in Neutrophils
|
|---|---|
| Chapter number | 8 |
| Book title |
Leucocytes
|
| Published in |
Methods in molecular biology, January 2012
|
| DOI | 10.1007/978-1-61779-527-5_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-526-8, 978-1-61779-527-5
|
| Authors |
Yu Chen, Wolfgang G. Junger, Chen, Yu, Junger, Wolfgang G. |
| Abstract |
Polymorphonuclear neutrophils (PMNs) generate reactive oxygen species (ROS) during phagocytosis and in response to soluble agonists. This functional response, termed oxidative burst, contributes to host defense, but it can also result in collateral damage of host tissues. To study this important PMN response, different methods have been developed that are based on the assessment of oxidative burst by measuring intracellular ROS production or formation of ROS in the extracellular space. Among the different methods that were developed, the following two are particularly widely used because of their convenience and accuracy. The first method depends on the reduction of cytochrome c, which can be assessed by photometry, while the second method relies on changes in the fluorescence properties of dihydrorhodamine 123, which can be assessed by flow cytometry. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Poland | 1 | <1% |
| France | 1 | <1% |
| Australia | 1 | <1% |
| Unknown | 232 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 51 | 22% |
| Student > Bachelor | 36 | 15% |
| Researcher | 34 | 14% |
| Student > Master | 26 | 11% |
| Student > Doctoral Student | 16 | 7% |
| Other | 25 | 11% |
| Unknown | 48 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 51 | 22% |
| Biochemistry, Genetics and Molecular Biology | 39 | 17% |
| Immunology and Microbiology | 36 | 15% |
| Medicine and Dentistry | 26 | 11% |
| Chemistry | 5 | 2% |
| Other | 24 | 10% |
| Unknown | 55 | 23% |