Report for: Leucocytes

You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.

Chapter title	Detection and quantification of cytokines and other biomarkers.
Chapter number	2
Book title	Leucocytes
Published in	Methods in molecular biology, January 2012
DOI	10.1007/978-1-61779-527-5_2
Pubmed ID	22262432
Book ISBNs	978-1-61779-526-8, 978-1-61779-527-5
Authors	Chiswick EL, Duffy E, Japp B, Remick D, Evan L. Chiswick, Elizabeth Duffy, Brian Japp, Daniel Remick, Chiswick, Evan L., Duffy, Elizabeth, Japp, Brian, Remick, Daniel
Abstract	Accurate measurement of cytokine concentrations is a powerful and essential approach to the study of inflammation. The enzyme-linked immunosorbent assay (ELISA) is a simple, low-cost analytical tool that provides both the specificity and sensitivity required for the study of cytokines in vitro or in vivo. This communication describes a systematic approach to develop an indirect sandwich ELISA to detect and quantify cytokines, or other biomarkers, with accuracy and precision. Also detailed is the use of sequential ELISA assays to analyze multiple cytokines from samples with limited volumes. Finally, the concept of a multiplex ELISA is discussed with considerations given to cost and additional time required for development.

View on publisher site Alert me about new mentions

Mendeley readers

The data shown below were compiled from readership statistics for 320 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	320	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Bachelor	59	18%
Student > Master	39	12%
Student > Ph. D. Student	32	10%
Researcher	23	7%
Student > Doctoral Student	13	4%
Other	27	8%
Unknown	127	40%

Readers by discipline	Count	As %
Biochemistry, Genetics and Molecular Biology	54	17%
Pharmacology, Toxicology and Pharmaceutical Science	24	8%
Medicine and Dentistry	21	7%
Immunology and Microbiology	14	4%
Chemistry	14	4%
Other	57	18%
Unknown	136	43%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2012.

All research outputs

#22,718,859

of 25,337,969 outputs

Outputs from Methods in molecular biology

#10,952

of 14,185 outputs

Outputs of similar age

#235,077

of 258,210 outputs

Outputs of similar age from Methods in molecular biology

#444

of 491 outputs

Altmetric has tracked 25,337,969 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.

So far Altmetric has tracked 14,185 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.

Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 258,210 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

We're also able to compare this research output to 491 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

Leucocytes

Table of Contents

Mentioned by

Readers on

Mendeley readers

Geographical breakdown

Demographic breakdown

Attention Score in Context