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Salamanders in Regeneration Research

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Cover of 'Salamanders in Regeneration Research'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Variation in Salamanders: An Essay on Genomes, Development, and Evolution.
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    Chapter 2 Maintaining Eastern Newts ( Notophthalmus viridescens ) for Regeneration Research
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    Chapter 3 Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl
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    Chapter 4 Husbandry of Spanish Ribbed Newts ( Pleurodeles waltl )
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    Chapter 5 Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies
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    Chapter 6 Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH.
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    Chapter 7 Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation
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    Chapter 8 The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration
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    Chapter 9 High-Efficiency Electroporation of the Spinal Cord in Larval Axolotl
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    Chapter 10 Pseudotyped Retroviruses for Infecting Axolotl
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    Chapter 11 Thyroxine-Induced Metamorphosis in the Axolotl (Ambystoma mexicanum).
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    Chapter 12 Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos
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    Chapter 13 In Vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.
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    Chapter 14 Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.
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    Chapter 15 Culture and Transfection of Axolotl Cells
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    Chapter 16 Isolation and Culture of Neurospheres from the Adult Newt Brain
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    Chapter 17 Methods for Axolotl Blood Collection, Intravenous Injection, and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb
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    Chapter 18 Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture
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    Chapter 19 Long-Term Organ Cultures of Newt Hearts
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    Chapter 20 In Vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration
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    Chapter 21 Transgenesis in Axolotl ( Ambystoma mexicanum )
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    Chapter 22 Generating and Identifying Axolotls with Targeted Mutations Using Cas9 RNA-Guided Nuclease.
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    Chapter 23 Gene Manipulation for Regenerative Studies Using the Iberian Ribbed Newt, Pleurodeles waltl
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    Chapter 24 Transcriptomics using axolotls.
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    Chapter 25 Sal-Site: Research Resources for the Mexican Axolotl
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    Chapter 26 Data Mining in Newt-Omics, the Repository for Omics Data from the Newt.
Attention for Chapter 13: In Vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.
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Chapter title
In Vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.
Chapter number 13
Book title
Salamanders in Regeneration Research
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2495-0_13
Pubmed ID
Book ISBNs
978-1-4939-2494-3, 978-1-4939-2495-0
Authors

Jami R Erickson, Karen Echeverri, Jami R. Erickson

Abstract

The ability to regenerate diseased, injured, or missing complex tissue is widespread throughout lower vertebrates and invertebrates; however, our knowledge of the molecular mechanisms that regulate this amazing ability is still in its infancy. Many recent papers have shown important roles for microRNAs in regulating regeneration in a number of species. The ability to detect and quantify miRNA expression fluctuations at a single cell level in vivo in different cell types during processes like regeneration is very informative. In this chapter, we describe how to use a dual-fluorescent green fluorescent protein (GFP)-reporter/monomeric red fluorescent protein (mRFP)-sensor (DFRS) plasmid to quantitate the dynamics of specific miRNAs over time following miRNA mimic injection as well as during regeneration. In this bicistronic vector, the mRFP allows for verification of miRNA expression, while the GFP functions as an internal control to normalize miRNA expression and thus obtain quantitative results. In addition, we demonstrate how this technique revealed dynamic miR-23a expression and function during tail regeneration.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Other 3 25%
Researcher 2 17%
Student > Bachelor 2 17%
Student > Master 2 17%
Librarian 1 8%
Other 1 8%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 33%
Computer Science 2 17%
Agricultural and Biological Sciences 2 17%
Medicine and Dentistry 1 8%
Neuroscience 1 8%
Other 0 0%
Unknown 2 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2015.
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#4,050,462
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Outputs from Methods in molecular biology
#2,333
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Outputs of similar age
#120,051
of 144,546 outputs
Outputs of similar age from Methods in molecular biology
#27
of 36 outputs
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