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Chapter title |
Membrane Nanotube Formation in Osteoclastogenesis
|
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Chapter number | 14 |
Book title |
Cell Fusion
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2703-6_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2702-9, 978-1-4939-2703-6
|
Authors |
Toshio Kukita, Akira Takahashi, Jing-Qi Zhang, Akiko Kukita, Kukita, Toshio, Takahashi, Akira, Zhang, Jing-Qi, Kukita, Akiko |
Abstract |
Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastogenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell-cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 1 | 33% |
Lecturer > Senior Lecturer | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Unknown | 1 | 33% |