| Chapter title |
Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2)
|
|---|---|
| Chapter number | 13 |
| Book title |
Histone Deacetylases
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3667-0_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3665-6, 978-1-4939-3667-0
|
| Authors |
Shaoping Ji, J. Ronald Doucette, Adil J. Nazarali, Ji, Shaoping, Doucette, J. Ronald, Nazarali, Adil J. |
| Abstract |
SIRT2 is a NAD(+)-dependent deacetylase that belongs to the sirtuin family, which is comprised of seven members (SIRT1-SIRT7) in humans. Furthermore, recent study shows that the Sirt2 gene has three transcript variants in mice. Several diverse proteins have been identified as SIRT2 substrates. SIRT2 activity involves multiple cell processes including growth, differentiation, and energy metabolism. However, little is known of SIRT2's role in oligodendrocytes or in the myelin sheath, where it is an important component. Here we describe procedures that detail Sirt2 gene cloning, identification, expression, and biological analysis in cultured cells. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 17% |
| Student > Postgraduate | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 1 | 17% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Neuroscience | 1 | 17% |
| Unknown | 3 | 50% |