Title |
Bone marrow-derived mesenchymal stem cells modulate BV2 microglia responses to lipopolysaccharide
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Published in |
International Immunopharmacology, September 2010
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DOI | 10.1016/j.intimp.2010.09.001 |
Pubmed ID | |
Authors |
Yin Yin Ooi, Rajesh Ramasamy, Zul'atfi Rahmat, Hemavathy Subramaiam, Shi Wei Tan, Maha Abdullah, Daud Ahmad Israf, Sharmili Vidyadaran |
Abstract |
The immunoregulatory properties of mesenchymal stem cells (MSC) have been demonstrated on a wide range of cells. Here, we describe the modulatory effects of mouse bone marrow-derived MSC on BV2 microglia proliferation rate, nitric oxide (NO) production and CD40 expression. Mouse bone marrow MSC were co-cultured with BV2 cells at various seeding density ratios and activated with lipopolysaccharide (LPS). We show that MSC exert an anti-proliferative effect on microglia and are potent producers of NO when stimulated by soluble factors released by LPS-activated BV2. MSC suppressed proliferation of both untreated and LPS-treated microglia in a dose-dependent manner, significantly reducing BV2 proliferation at seeding density ratios of 1:0.2 and 1:0.1 (p<.05). Co-culturing MSC with BV2 cells at different ratios revealed interesting dynamics in NO production. A high number of MSC significantly increases NO in co-cultures whilst a lower number reduces NO. The increased NO levels in co-cultures may be MSC-derived, as we also show that activated BV2 cells stimulate MSC to produce NO. Cell-cell interaction is not a requirement for this effect as soluble factors released by activated BV2 cells alone do stimulate MSC to produce high levels of NO. Although NO is implicated as a mediator for T cell proliferation, it does not appear to play a major role in the suppression of microglia proliferation. Additionally, MSC reduced the expression of the microglial co-stimulator molecule, CD40. Collectively, these regulatory effects of MSC on microglia offer insight into the potential moderating properties of MSC on inflammatory responses within the CNS. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 52 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 14 | 27% |
Researcher | 9 | 17% |
Student > Master | 5 | 10% |
Student > Bachelor | 4 | 8% |
Professor > Associate Professor | 4 | 8% |
Other | 8 | 15% |
Unknown | 8 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 15 | 29% |
Medicine and Dentistry | 9 | 17% |
Biochemistry, Genetics and Molecular Biology | 5 | 10% |
Neuroscience | 4 | 8% |
Immunology and Microbiology | 3 | 6% |
Other | 4 | 8% |
Unknown | 12 | 23% |