Chapter title |
Split-Transgene Expression in Wheat
|
---|---|
Chapter number | 11 |
Book title |
Transgenic Plants
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-558-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-61779-557-2, 978-1-61779-558-9
|
Authors |
Mario Gils, Myroslava Rubtsova, Katja Kempe, Gils, Mario, Rubtsova, Myroslava, Kempe, Katja |
Abstract |
The establishment of traits that result from the concerted expression of complementing transgene fragments is a feasible tool for trait control or gene flow control in plants. This chapter describes the methodology for producing herbicide-resistant and pollen-sterile wheat plants by the intein-mediated assembly of inactive precursor protein fragments (protein trans-splicing). We suggest the design of intein-containing vectors for split-transgene expression. We describe transient plant assays that can be used to analyse the functionality of the system and describe the transformation of wheat plants using a split selection marker.We hope that this chapter will be a helpful guideline for researchers who are interested in applying similar split-gene approaches in wheat or other monocotyledonous crops. |
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Demographic breakdown
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Researcher | 4 | 31% |
Librarian | 2 | 15% |
Student > Master | 2 | 15% |
Student > Bachelor | 1 | 8% |
Unspecified | 1 | 8% |
Other | 0 | 0% |
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Unknown | 3 | 23% |