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How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?

Overview of attention for article published in RNA, March 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#1 of 3,207)
  • High Attention Score compared to outputs of the same age (98th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

Readers on

mendeley
2068 Mendeley
citeulike
13 CiteULike
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Title
How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?
Published in
RNA, March 2016
DOI 10.1261/rna.053959.115
Pubmed ID
Authors

Nicholas J. Schurch, Pietá Schofield, Marek Gierliński, Christian Cole, Alexander Sherstnev, Vijender Singh, Nicola Wrobel, Karim Gharbi, Gordon G. Simpson, Tom Owen-Hughes, Mark Blaxter, Geoffrey J. Barton

X Demographics

X Demographics

The data shown below were collected from the profiles of 217 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2,068 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 13 <1%
Germany 5 <1%
Spain 4 <1%
Brazil 4 <1%
United Kingdom 4 <1%
Japan 3 <1%
Denmark 2 <1%
Portugal 1 <1%
Sweden 1 <1%
Other 12 <1%
Unknown 2019 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 493 24%
Researcher 431 21%
Student > Master 241 12%
Student > Bachelor 186 9%
Student > Doctoral Student 109 5%
Other 270 13%
Unknown 338 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 652 32%
Biochemistry, Genetics and Molecular Biology 612 30%
Medicine and Dentistry 86 4%
Immunology and Microbiology 66 3%
Computer Science 49 2%
Other 216 10%
Unknown 387 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 172. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 November 2023.
All research outputs
#238,698
of 25,837,817 outputs
Outputs from RNA
#1
of 3,207 outputs
Outputs of similar age
#4,240
of 317,674 outputs
Outputs of similar age from RNA
#1
of 34 outputs
Altmetric has tracked 25,837,817 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,207 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,674 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.