Chapter title |
Isolation of melanoma tumor-initiating cells from surgical tissues.
|
---|---|
Chapter number | 16 |
Book title |
Molecular Dermatology
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-227-8_16 |
Pubmed ID | |
Book ISBNs |
978-1-62703-226-1, 978-1-62703-227-8
|
Authors |
Boiko AD, Alexander D. Boiko |
Abstract |
A new model of cancer progression has been put forward that predicts existence of tumor stem cells (TSCs) in the heterogeneous bulk tumor mass that self-renew, are resistant to chemo- and radiotherapies, and sustain tumor growth during the course of its progression or relapse (Ailles and Weissman, Curr Opin Biotechnol 18:460-466, 2007; Chan et al., Proc Natl Acad Sci U S A 106:14016-14021, 2009; D'Angelo and Wicha, Prog Mol Biol Transl Sci 95:113-158, 2010; O'Brien, Semin Radiat Oncol 19:71-77, 2009; Park et al., Mol Ther 17:219-230, 2009). Using most advanced methods of cell purification and transplantation, our laboratory and another independent study identified melanoma stem cells as CD271(NFGR/p75)+ cells from surgical human specimens (Boiko et al., Nature 466:133-137, 2010; Civenni et al., Cancer Res 71:3098-3109, 2011). Here we describe in great detail an approach for isolating tumor-initiating cells from freshly resected melanomas (Boiko et al., Nature 466:133-137, 2010). |
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United States | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 16 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 5 | 31% |
Researcher | 3 | 19% |
Student > Master | 2 | 13% |
Professor | 1 | 6% |
Other | 1 | 6% |
Other | 2 | 13% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 25% |
Agricultural and Biological Sciences | 4 | 25% |
Medicine and Dentistry | 3 | 19% |
Immunology and Microbiology | 1 | 6% |
Materials Science | 1 | 6% |
Other | 1 | 6% |
Unknown | 2 | 13% |