| Chapter title |
Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization
|
|---|---|
| Chapter number | 2 |
| Book title |
The Nuclear Receptor Superfamily
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3724-0_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3722-6, 978-1-4939-3724-0
|
| Authors |
Jean-Marc A. Lobaccaro, Claude Beaudoin, Bagora Bayala, Silvère Baron, Amalia Trousson, Lobaccaro, Jean-Marc A., Beaudoin, Claude, Bayala, Bagora, Baron, Silvère, Trousson, Amalia |
| Abstract |
Screening of bona fide ligands for nuclear receptors is a real tour de force as the identified molecules are supposed to be able to activate the targeted proteins in cell culture as well as in vivo. Indeed orphan nuclear receptors are putative pharmacologically targets for various diseases. It is thus necessary to have quick and reproductive systems that help in identifying new ligands, agonist or antagonist, before using them in vivo in animal models to check for secondary effects. Here, we describe the transient transfections (homologous and heterologous) used for the screening of ligands for liver X receptor α (LXRα, NR1H3) in HeLa cells. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 3 | 75% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |
| Unknown | 2 | 50% |