Chapter title |
Methods for Identifying and Quantifying mRNA Expression of Androgen Receptor Splicing Variants in Prostate Cancer
|
---|---|
Chapter number | 11 |
Book title |
The Nuclear Receptor Superfamily
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3724-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3722-6, 978-1-4939-3724-0
|
Authors |
Yingming Li, Scott M. Dehm, Li, Yingming, Dehm, Scott M. |
Abstract |
Constitutively active androgen receptor (AR) variants (AR-Vs) lacking the AR ligand-binding domain have been identified as drivers of prostate cancer resistance to AR-targeted therapies. A definitive understanding of the role and origin of AR-Vs in the natural history of prostate cancer progression requires cataloging the entire spectrum of AR-Vs expressed in prostate cancer, as well as accurate determination of their expression levels relative to full-length AR in clinical tissues and models of progression. Exon constituency differences at the 3' terminus of mRNAs encoding AR-Vs compared with mRNAs encoding full-length AR can be exploited for discovery and quantification-based experiments. Here, we provide methodological details for 3' rapid amplification of cDNA ends (3' RACE) and absolute quantitative RT-PCR, which are cost-effective approaches for identifying new AR-Vs and quantifying their absolute expression levels in conjunction with full-length AR in RNA samples derived from various sources. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 2 | 33% |
Other | 2 | 33% |
Student > Doctoral Student | 1 | 17% |
Unknown | 1 | 17% |
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Biochemistry, Genetics and Molecular Biology | 1 | 17% |
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Medicine and Dentistry | 1 | 17% |
Unknown | 1 | 17% |