| Chapter title |
Cultured ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses
|
|---|---|
| Chapter number | 14 |
| Book title |
Handbook of ELISPOT
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8567-8_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8566-1, 978-1-4939-8567-8
|
| Authors |
Chandima Jeewandara, Graham S. Ogg, Gathsaurie Neelika Malavige, Jeewandara, Chandima, Ogg, Graham S., Malavige, Gathsaurie Neelika |
| Abstract |
The cultured Enzyme-Linked ImmunoSpot (ELISpot) assay is a functional T cell assay, which is commonly used to assess virus-specific T cell responses. The use of an in vitro expansion step before the ELISpot distinguishes such "cultured" ELISpots from "ex vivo" ELISpots. Cultured ELISpots have the advantage that lower frequency responses can be analyzed compared to ex vivo ELISpots, but do carry the associated potential distortions of the expansion phase. Cultured ELISpot assays are of value to determine silent and symptomatic transmission of the Dengue virus (DENV) in the community and to identify the correlates of a DENV-specific protective immune response. We have evaluated T cell responses to the DENV using cultured ELISpot assays with serotype-specific T cell epitopes to determine past infecting dengue virus (DENV) serotypes. The peptides used in this assay do not cross react with the Japanese encephalitis virus nor other flaviviruses. Therefore, this assay is likely to be useful in determining the past infecting DENV serotype in immune-epidemiological studies and in dengue vaccine trials. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 17% |
| Student > Doctoral Student | 3 | 13% |
| Student > Ph. D. Student | 3 | 13% |
| Student > Master | 3 | 13% |
| Student > Postgraduate | 2 | 8% |
| Other | 3 | 13% |
| Unknown | 6 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 25% |
| Medicine and Dentistry | 4 | 17% |
| Agricultural and Biological Sciences | 1 | 4% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Social Sciences | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 10 | 42% |