Chapter title |
Challenges in Developing Protein Secretion Assays at a Single-Cell Level
|
---|---|
Chapter number | 1 |
Book title |
Handbook of ELISPOT
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8567-8_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8566-1, 978-1-4939-8567-8
|
Authors |
Yoshitaka Shirasaki, Osamu Ohara, Shirasaki, Yoshitaka, Ohara, Osamu |
Abstract |
In addition to direct physical interactions between/among cells, the secretion of humoral factors from living cells is a critical process for cell-cell communications. A well-known extracellular signaling event is mediated by immune cell cytokines/chemokines. Because cell-cell communication is crucial in immune cell sociology, protein secretion assays first attracted a broad range of immunology interests. Now that we have entered an era of systems biology, cell-cell interactions mediated by secreted molecules should be revisited to understand the dynamics and homeostasis of the cell society as a whole. Of more importance, recent advances in detection and microfluidics technologies enable us to monitor protein secretion in real time rather than as a snapshot from the past, which gives us an opportunity to more deeply understand the logic of mammalian cell sociology. This chapter reviews the recent progress in and future direction of protein secretion assays, particularly from a mammalian cell sociology viewpoint. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 29% |
Researcher | 2 | 29% |
Student > Bachelor | 1 | 14% |
Professor > Associate Professor | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Chemical Engineering | 1 | 14% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Immunology and Microbiology | 1 | 14% |
Materials Science | 1 | 14% |
Other | 0 | 0% |
Unknown | 2 | 29% |