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Handbook of ELISPOT

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Cover of 'Handbook of ELISPOT'

Table of Contents

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    Book Overview
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    Chapter 1 Challenges in Developing Protein Secretion Assays at a Single-Cell Level
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    Chapter 2 Mastering the Computational Challenges of Elispot Plate Evaluation
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    Chapter 3 Essential Controls for ELISpot Assay
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    Chapter 4 Automatic Search of Spots and Color Classification in ELISPOT Assay
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    Chapter 5 Four Color ImmunoSpot ® Assays for Identification of Effector T-Cell Lineages
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    Chapter 6 Detection of Cross-Reactive B Cells Using the FluoroSpot Assay
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    Chapter 7 Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations
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    Chapter 8 Detecting all Immunoglobulin Classes and Subclasses in a Multiplex 7 Color ImmunoSpot® Assay
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    Chapter 9 Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot ® Software Based on Center of Mass Distance Algorithm
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    Chapter 10 Multi-Color FLUOROSPOT Counting Using ImmunoSpot ® Fluoro-X™ Suite
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    Chapter 11 B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells
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    Chapter 12 Identification of Novel Mycobacterial Targets for Murine CD4 + T-Cells by IFNγ ELISPOT
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    Chapter 13 ELISPOT-Based “Multi-Color FluoroSpot” to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections
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    Chapter 14 Cultured ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses
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    Chapter 15 Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses
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    Chapter 16 Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection
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    Chapter 17 Dendritic Cell-Based ELISpot Assay for Assessing T-Cell IFN-γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins
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    Chapter 18 Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccine
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    Chapter 19 Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs Using ELISpot Assay
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    Chapter 20 Towards a Full Automation of the ELISpot Assay for Safe and Parallelized Immunomonitoring
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