Title |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
|
---|---|
Published in |
Brazilian Journal of Microbiology, September 2015
|
DOI | 10.1590/s1517-838246320140556 |
Pubmed ID | |
Authors |
Xufeng Han, Lei Wang, Wei Li, Bibo Li, Yuxin Yang, Hailong Yan, Lei Qu, Yulin Chen |
Abstract |
The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Argentina | 1 | 4% |
Unknown | 25 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 9 | 35% |
Student > Ph. D. Student | 5 | 19% |
Researcher | 2 | 8% |
Professor | 1 | 4% |
Student > Doctoral Student | 1 | 4% |
Other | 2 | 8% |
Unknown | 6 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 23% |
Agricultural and Biological Sciences | 5 | 19% |
Environmental Science | 2 | 8% |
Medicine and Dentistry | 2 | 8% |
Nursing and Health Professions | 1 | 4% |
Other | 3 | 12% |
Unknown | 7 | 27% |