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Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats

Overview of attention for article published in Brazilian Journal of Microbiology, September 2015
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  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

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Title
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
Published in
Brazilian Journal of Microbiology, September 2015
DOI 10.1590/s1517-838246320140556
Pubmed ID
Authors

Xufeng Han, Lei Wang, Wei Li, Bibo Li, Yuxin Yang, Hailong Yan, Lei Qu, Yulin Chen

Abstract

The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Argentina 1 4%
Unknown 25 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 35%
Student > Ph. D. Student 5 19%
Researcher 2 8%
Student > Doctoral Student 1 4%
Professor 1 4%
Other 3 12%
Unknown 5 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 23%
Agricultural and Biological Sciences 5 19%
Environmental Science 2 8%
Medicine and Dentistry 2 8%
Unspecified 1 4%
Other 4 15%
Unknown 6 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 September 2023.
All research outputs
#16,721,717
of 25,374,647 outputs
Outputs from Brazilian Journal of Microbiology
#529
of 1,377 outputs
Outputs of similar age
#156,817
of 276,791 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#8
of 25 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,377 research outputs from this source. They receive a mean Attention Score of 3.6. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 276,791 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.