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Chapter title |
A Practical Protocol for the Conditional Depletion of Rho Isoforms in Human Embryonic Stem Cells
|
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Chapter number | 20 |
Book title |
Rho GTPases
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8612-5_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
|
Authors |
Masatoshi Ohgushi, Ohgushi, Masatoshi |
Abstract |
Human pluripotent stem cells indefinitely proliferate and survive in culture while retaining genomic integrity, providing a unique opportunity to study human molecular biology. Here, we introduced an RNA interference-based protocol of inducible gene silencing in human embryonic stem cells, which has several advantages in handling simplicity/convenience, cost/time performance, and applicability. Using this method, we had succeeded to elucidate the isoform-unique roles of Rho-family small GTPases in human embryonic stem cells. |