Chapter title |
Measuring the Contributions of the Rho Pathway to the DNA Damage Response in Tumor Epithelial Cells
|
---|---|
Chapter number | 23 |
Book title |
Rho GTPases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8612-5_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8611-8, 978-1-4939-8612-5
|
Authors |
Yuli T. Magalhães, Jessica O. Farias, Lucas F. Monteiro, Fábio L. Forti, Magalhães, Yuli T., Farias, Jessica O., Monteiro, Lucas F., Forti, Fábio L. |
Abstract |
Actin polymerization, actomyosin ring contraction, and stress fiber formation are examples of relevant actions of the RhoA/B/C pathway as GTPases that regulate the cytoskeleton. However, open questions that remain to be addressed are whether this pathway and/or downstream components protect against or facilitate the formation of DNA double-strand breaks, the most lethal form of DNA damage in cells. Genotoxic drugs are radiomimetic and/or chemotherapeutic agents that are currently used for cancer treatments and are associated with specific methodologies; thus, these compounds should represent good tools to answer these questions. In this chapter, we describe two methods, the alkaline comet assay and homologous/nonhomologous recombination assays, to investigate the mechanism by which the Rho pathway modulates the repair of DNA breaks in tumor epithelial cell lines. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 36% |
Student > Bachelor | 3 | 27% |
Other | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 55% |
Chemistry | 2 | 18% |
Agricultural and Biological Sciences | 1 | 9% |
Unknown | 2 | 18% |