| Chapter title |
Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides
|
|---|---|
| Chapter number | 13 |
| Book title |
Microbial Carotenoids
|
| Published in |
Methods in molecular biology, August 2018
|
| DOI | 10.1007/978-1-4939-8742-9_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8741-2, 978-1-4939-8742-9
|
| Authors |
Francisco E. Nicolás, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Sergio López-García, Eusebio Navarro, Santiago Torres-Martínez, Victoriano Garre, Nicolás, Francisco E., Navarro-Mendoza, María Isabel, Pérez-Arques, Carlos, López-García, Sergio, Navarro, Eusebio, Torres-Martínez, Santiago, Garre, Victoriano |
| Abstract |
The carotene producer Mucor circinelloides is the fungus within the Mucoromycota phylum with the widest repertoire of molecular tools to manipulate its genome. The initial development of an effective procedure for genetic transformation and later improvements have resulted in an expansion of available tools, which include gene replacement, inactivation of gene expression by RNA silencing, gene overexpression, and functional genomics. Moreover, sequencing of its genome has given a definitive boost to these techniques making attainable the study of genes involved in many physiological or developmental processes, including carotenoid biosynthesis. Here, we describe in detail the latest molecular techniques currently used in M. circinelloides that have made it a valuable model for studying gene function within its phylum. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 15% |
| Student > Ph. D. Student | 3 | 12% |
| Student > Doctoral Student | 2 | 8% |
| Researcher | 2 | 8% |
| Student > Bachelor | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 13 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 35% |
| Agricultural and Biological Sciences | 3 | 12% |
| Chemical Engineering | 1 | 4% |
| Unknown | 13 | 50% |