Chapter title |
Imaging Platelets and Megakaryocytes by High-Resolution Laser Fluorescence Microscopy
|
---|---|
Chapter number | 2 |
Book title |
Platelets and Megakaryocytes
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8585-2_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8584-5, 978-1-4939-8585-2
|
Authors |
Pluthero, Fred G, Kahr, Walter H A, Fred G. Pluthero, Walter H. A. Kahr |
Abstract |
Microscopy is central to studies of platelets and their precursor megakaryocytes. Here we describe methods to rapidly obtain high resolution images of fixed platelets, megakaryocytes and megakaryocytic cells via immunofluorescence microscopy. Protocols covered include: (1) isolation and preparation of cells suitable for fluorescence staining; (2) staining with antibodies and other molecules; (3) imaging via spinning-disc confocal and structured illumination laser fluorescence microscopy; (4) processing and presentation of images. Also included is a list of primary antibodies we have validated for use in staining specific proteins and subcellular structures in platelets and megakaryocytes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 33% |
Other | 1 | 11% |
Professor | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Student > Master | 1 | 11% |
Other | 1 | 11% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Chemical Engineering | 1 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Biochemistry, Genetics and Molecular Biology | 1 | 11% |
Agricultural and Biological Sciences | 1 | 11% |
Immunology and Microbiology | 1 | 11% |
Other | 3 | 33% |
Unknown | 1 | 11% |