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Mendeley readers
| Chapter title |
Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor-Driven Forward Programming
|
|---|---|
| Chapter number | 10 |
| Book title |
Platelets and Megakaryocytes
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8585-2_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8584-5, 978-1-4939-8585-2
|
| Authors |
Thomas Moreau, Amanda L. Evans, Cedric J. G. Ghevaert |
| Abstract |
The differentiation of megakaryocytes from human pluripotent stem cells in vitro offers intriguing new perspectives for research and transfusion medicine. However, applications have been hampered by the low efficiency of cytokine driven differentiation protocols leading to poor megakaryocyte purity and yield. Here we describe a novel forward programming approach relying on the combined ectopic expression of the three transcription factors GATA1, FLI1, and TAL1 in human pluripotent stem cells for large scale production of mature megakaryocytes using chemically defined culture and minimum cytokines. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 31% |
| Researcher | 3 | 23% |
| Student > Master | 2 | 15% |
| Student > Ph. D. Student | 1 | 8% |
| Unknown | 3 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 54% |
| Agricultural and Biological Sciences | 2 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Unknown | 2 | 15% |