Chapter title |
Biofilms and Antifungal Susceptibility Testing.
|
---|---|
Chapter number | 14 |
Book title |
Candida Species
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3052-4_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3051-7, 978-1-4939-3052-4
|
Authors |
Simitsopoulou, Maria, Chatzimoschou, Athanasios, Roilides, Emmanuel, Maria Simitsopoulou, Athanasios Chatzimoschou, Emmanuel Roilides |
Abstract |
Yeasts and filamentous fungi both exist as single cells and hyphal forms, two morphologies used by most fungal organisms to create a complex multilayered biofilm structure. In this chapter we describe the most widely used assays for the determination of biofilm production and assessment of susceptibility of biofilms to antifungal agents or host phagocytes as various methods, the most frequent of which are staining, confocal laser scanning microscopy, quantification of extracellular DNA and protein associated with extracellular matrix and XTT metabolic reduction assay. Pathway-focused biofilm gene expression profiling is assessed by real-time reverse transcriptase polymerase chain reaction. |
X Demographics
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France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
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Indonesia | 1 | 8% |
Brazil | 1 | 8% |
Unknown | 11 | 85% |
Demographic breakdown
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Student > Master | 4 | 31% |
Other | 1 | 8% |
Lecturer | 1 | 8% |
Professor > Associate Professor | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Other | 3 | 23% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 3 | 23% |
Medicine and Dentistry | 2 | 15% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Chemical Engineering | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |