| Chapter title |
Culturing Drosophila Egg Chambers and Investigating Developmental Processes Through Live Imaging
|
|---|---|
| Chapter number | 5 |
| Book title |
Drosophila Oogenesis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2851-4_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2850-7, 978-1-4939-2851-4
|
| Authors |
Lathiena Manning, Michelle Starz-Gaiano |
| Abstract |
Drosophila oogenesis provides many examples of essential processes in development. A myriad of genetic tools combined with recent advances in culturing egg chambers ex vivo has revealed several surprising mechanisms that govern how this tissue develops, and which could not have been determined in fixed tissues. Here we describe a straightforward protocol for dissecting ovaries, culturing egg chambers, and observing egg development in real time by fluorescent microscopy. This technique is suitable for observation of early- or late-stage egg development, and can be adapted to study a variety of cellular, molecular, or developmental processes. Ongoing analysis of oogenesis in living egg chambers has tremendous potential for discovery of new developmental mechanisms. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 50% |
| Professor | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Medicine and Dentistry | 1 | 25% |
| Unknown | 1 | 25% |