Title |
Efficient subtraction of insect rRNA prior to transcriptome analysis of Wolbachia-Drosophila lateral gene transfer
|
---|---|
Published in |
BMC Research Notes, May 2012
|
DOI | 10.1186/1756-0500-5-230 |
Pubmed ID | |
Authors |
Nikhil Kumar, Todd Creasy, Yezhou Sun, Melissa Flowers, Luke J Tallon, Julie C Dunning Hotopp |
Abstract |
Numerous methods exist for enriching bacterial or mammalian mRNA prior to transcriptome experiments. Yet there persists a need for methods to enrich for mRNA in non-mammalian animal systems. For example, insects contain many important and interesting obligate intracellular bacteria, including endosymbionts and vector-borne pathogens. Such obligate intracellular bacteria are difficult to study by traditional methods. Therefore, genomics has greatly increased our understanding of these bacteria. Efficient subtraction methods are needed for removing both bacteria and insect rRNA in these systems to enable transcriptome-based studies. |
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Country | Count | As % |
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United States | 1 | 17% |
Unknown | 5 | 83% |
Demographic breakdown
Type | Count | As % |
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Scientists | 4 | 67% |
Members of the public | 2 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 2% |
United Kingdom | 2 | 2% |
Brazil | 1 | 1% |
Czechia | 1 | 1% |
New Zealand | 1 | 1% |
Canada | 1 | 1% |
Unknown | 87 | 92% |
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Readers by professional status | Count | As % |
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Researcher | 30 | 32% |
Student > Ph. D. Student | 22 | 23% |
Student > Master | 12 | 13% |
Professor | 7 | 7% |
Professor > Associate Professor | 5 | 5% |
Other | 10 | 11% |
Unknown | 9 | 9% |
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Environmental Science | 3 | 3% |
Nursing and Health Professions | 1 | 1% |
Other | 6 | 6% |
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