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Mendeley readers
Chapter title |
Assaying ATE1 Activity In Vitro.
|
---|---|
Chapter number | 10 |
Book title |
Protein Arginylation
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2935-1_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2934-4, 978-1-4939-2935-1
|
Authors |
Junling Wang, Anna S. Kashina |
Editors |
Anna S. Kashina |
Abstract |
Here we describe a standard arginyltransferase assay in vitro using bacterially expressed purified ATE1 in a system with minimal number of components (Arg, tRNA, Arg-tRNA synthetase, and arginylation substrate). Assays of this type have first been developed in the 1980s using crude ATE1 preparations from cells and tissues and then perfected recently for the use with bacterially expressed recombinant protein. This assay represents a simple and efficient way to measure ATE1 activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 25% |
Student > Ph. D. Student | 1 | 25% |
Lecturer | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 50% |
Agricultural and Biological Sciences | 1 | 25% |
Unknown | 1 | 25% |