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Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation

Overview of attention for article published in Animal Science Journal, June 2016
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Title
Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation
Published in
Animal Science Journal, June 2016
DOI 10.1111/asj.12634
Pubmed ID
Authors

Saeid Jafari, Yong M Goh, Mohamed A Rajion, Mohammad F Jahromi, Yusof H Ahmad, Mahdi Ebrahimi

Abstract

Papaya leaf methanolic extract (PLE) at concentrations of 0 (CON), 5 (LLE), 10 (MLE) and 15 (HLE) mg/250 mg dry matter (DM) with 30 mL buffered rumen fluid were incubated for 24 h to identify its effect on in vitro ruminal methanogenesis and ruminal biohydrogenation (BH). Total gas production was not affected (P > 0.05) by addition of PLE compared to the CON at 24 h of incubation. Methane (CH4 ) production (mL/250 mg DM) decreased (P < 0.05) with increasing levels of PLE. Acetate to propionate ratio was lower (P <0.05) in MLE (2.02) and HLE (1.93) compared to the CON (2.28). Supplementation of the diet with PLE significantly (P <0.05) decreased the rate of BH of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), C18:3n-3 (linolenic acid; LNA) and C18 polyunsaturated fatty acids (PUFA) compared to CON after 24 h incubation, which resulted in higher concentrations of BH intermediates such as C18:1 t11 (vaccenic acid; VA), c9t11 conjugated LA (CLA) (rumenic acid; RA) and t10c12 CLA. Real-time PCR analysis indicated that the total bacteria, total protozoa, Butyrivibrio fibrisolvens and methanogen population in HLE decreased (P <0.05) compared to CON, but the total bacteria and B. fibrisolvens population were higher (P < 0.05) in CON compared to the PLE treatment groups.

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Mendeley readers

The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 55 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 18%
Student > Master 8 15%
Lecturer 6 11%
Student > Doctoral Student 6 11%
Student > Ph. D. Student 5 9%
Other 8 15%
Unknown 12 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 56%
Medicine and Dentistry 4 7%
Nursing and Health Professions 3 5%
Veterinary Science and Veterinary Medicine 1 2%
Unspecified 1 2%
Other 2 4%
Unknown 13 24%