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Gap Junction Protocols

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Cover of 'Gap Junction Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.
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    Chapter 2 Gap Junction Protocols
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    Chapter 3 Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.
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    Chapter 4 Immunohisto- and Cytochemistry Analysis of Connexins.
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    Chapter 5 Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.
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    Chapter 6 Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor.
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    Chapter 7 Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells.
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    Chapter 8 Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis.
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    Chapter 9 Scrape Loading/Dye Transfer Assay.
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    Chapter 10 Microinjection Technique for Assessment of Gap Junction Function.
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    Chapter 11 Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.
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    Chapter 12 Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro.
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    Chapter 13 Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Imaging.
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    Chapter 14 Gap Junction Protocols
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    Chapter 15 Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels.
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    Chapter 16 Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
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Chapter title
Gap Junction Protocols
Chapter number 14
Book title
Gap Junction Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3664-9_14
Pubmed ID
Book ISBNs
978-1-4939-3662-5, 978-1-4939-3664-9
Authors

Stains, Joseph P, Civitelli, Roberto, Joseph P. Stains, Roberto Civitelli

Editors

Mathieu Vinken, Scott R. Johnstone

Abstract

Cell-to-cell transfer of small molecules is a fundamental way by which multicellular organisms coordinate function. Recent work has highlighted the complexity of biologic responses downstream of gap junctions. As the connexin-regulated effectors are coming into focus, there is a need to develop functional assays that allow specific testing of biologically relevant second messengers. Here, we describe a modification of the classic gap junction parachute assay to assess biologically relevant molecules passed through gap junctions.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 33%
Student > Doctoral Student 2 13%
Student > Bachelor 2 13%
Professor 1 7%
Other 1 7%
Other 2 13%
Unknown 2 13%
Readers by discipline Count As %
Medicine and Dentistry 5 33%
Biochemistry, Genetics and Molecular Biology 4 27%
Agricultural and Biological Sciences 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Computer Science 1 7%
Other 1 7%
Unknown 1 7%