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Mendeley readers
| Chapter title |
Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.
|
|---|---|
| Chapter number | 1 |
| Book title |
Gap Junction Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3664-9_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3662-5, 978-1-4939-3664-9
|
| Authors |
Michaël Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, Mathieu Vinken |
| Editors |
Mathieu Vinken, Scott R. Johnstone |
| Abstract |
Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin RNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction, and data analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 33% |
| Student > Bachelor | 2 | 22% |
| Student > Master | 2 | 22% |
| Professor | 1 | 11% |
| Researcher | 1 | 11% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 33% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 22% |
| Veterinary Science and Veterinary Medicine | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Neuroscience | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |