Molecular Motors
Springer New York
| Chapter title |
DNA Organization and Superesolved Segregation
|
|---|---|
| Chapter number | 14 |
| Book title |
Molecular Motors
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8556-2_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8554-8, 978-1-4939-8556-2
|
| Authors |
Diego I. Cattoni, Jean-Bernard Fiche, Antoine Le Gall, Marcelo Nollmann |
| Abstract |
With single-molecule localization microscopy (SMLM) it is possible to reveal the internal composition, architecture, and dynamics of molecular machines and large cellular complexes. SMLM remains technically challenging, and frequently its implementation requires tailored experimental conditions that depend on the complexity of the subcellular structure of interest. Here, we describe two simple, robust, and high-throughput protocols to study molecular motors and machineries responsible for chromosome transport and organization in bacteria using 2D- and 3D-SMLM. |
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 2 | 17% |
| Researcher | 2 | 17% |
| Student > Bachelor | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Professor > Associate Professor | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 5 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Physics and Astronomy | 1 | 8% |
| Unknown | 5 | 42% |