Chapter title |
High-Resolution Imaging of Cellular Proteins
|
---|---|
Chapter number | 15 |
Book title |
High-Resolution Imaging of Cellular Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6352-2_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6350-8, 978-1-4939-6352-2
|
Authors |
Fišerová, Jindřiška, Richardson, Christine, Goldberg, Martin W, Jindřiška Fišerová, Christine Richardson, Martin W. Goldberg |
Editors |
Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski |
Abstract |
Immunolabeling electron microscopy is a challenging technique with demands for perfect ultrastructural and antigen preservation. High-pressure freezing offers an excellent way to fix cellular structure. However, its use for immunolabeling has remained limited because of the low frequency of labeling due to loss of protein antigenicity or accessibility. Here we present a protocol for immunogold labeling of the yeast Saccharomyces cerevisiae that gives specific and multiple labeling while keeping the finest structural details. We use the protocol to reveal the organization of individual nuclear pore complex proteins and the position of transport factors in the yeast Saccharomyces cerevisiae in relation to actual transport events. |
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Mendeley readers
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