Chapter title |
Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues.
|
---|---|
Chapter number | 14 |
Book title |
High-Resolution Imaging of Cellular Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6352-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6350-8, 978-1-4939-6352-2
|
Authors |
Miyuki Takeuchi, Keiji Takabe, Yoshinobu Mineyuki |
Editors |
Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski |
Abstract |
Cryofixation and freeze-substitution techniques provide excellent preservation of plant ultrastructure. The advantage of cryofixation is not only in structural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more detailed information on the immunoelectron-microscopic localization of molecules in the plant cell than can be obtained from chemically fixed tissues. Here, we introduce methods for immunoelectron microscopy of cryofixed and freeze-substituted plant tissues. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 29% |
Student > Doctoral Student | 2 | 14% |
Professor > Associate Professor | 2 | 14% |
Student > Ph. D. Student | 1 | 7% |
Other | 1 | 7% |
Other | 2 | 14% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 50% |
Materials Science | 2 | 14% |
Computer Science | 1 | 7% |
Chemistry | 1 | 7% |
Engineering | 1 | 7% |
Other | 0 | 0% |
Unknown | 2 | 14% |