Chapter title |
Using FRAP or FRAPA to Visualize the Movement of Fluorescently Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses.
|
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Chapter number | 8 |
Book title |
High-Resolution Imaging of Cellular Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6352-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6350-8, 978-1-4939-6352-2
|
Authors |
Yaara Tevet, Daniel Gitler |
Editors |
Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski |
Abstract |
Fluorescence recovery after photobleaching (FRAP) and fluorescence redistribution after photoactivation (FRAPA) are complementary methods used to gauge the movement of proteins or sub-resolution organelles within cells. Using these methods we can determine the nature of the movement of labeled particles, whether it is random, constrained, or active, the coefficient of diffusion if applicable, binding and unbinding constants, and the direction of active transport. These two techniques have been extensively utilized to probe the cell biology of neurons. A practical outline of FRAP and FRAPA in cultured neurons is presented, including the preparation of the neurons and their infection with adeno-associated viral vectors. Considerations in planning such experiments are provided. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 36% |
Student > Master | 4 | 29% |
Student > Doctoral Student | 1 | 7% |
Other | 1 | 7% |
Student > Postgraduate | 1 | 7% |
Other | 0 | 0% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 3 | 21% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Agricultural and Biological Sciences | 1 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Other | 1 | 7% |
Unknown | 3 | 21% |