Chapter title |
High-Throughput RNAi Screening
|
---|---|
Chapter number | 1 |
Book title |
High-Throughput RNAi Screening
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6337-9_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6335-5, 978-1-4939-6337-9
|
Authors |
Yin, Hongwei, Sereduk, Chris, Tang, Nanyun, Hongwei Yin Ph.D., Chris Sereduk, Nanyun Tang, Hongwei Yin |
Editors |
David O. Azorsa, Shilpi Arora |
Abstract |
RNA interference (RNAi) is a readily available research tool that can be used to accelerate the identification and functional validation of a multitude of new candidate drug targets by experimentally perturbing gene expression and function. High-throughput RNAi technology using libraries of short-interfering RNA (siRNA) makes it possible to rapidly identify genes and biomarkers associated with biological processes such as diseases or a cellular response to therapy. Thus, RNAi-based screening is an extremely powerful technology that can provide tremendous insights into the mechanisms of action and contexts of vulnerability of a particular drug treatment. This chapter describes the infrastructure requirements needed to successfully perform HT-RNAi screening. Information on the methodology, instrumentation, experimental design, and workflow aspects is provided, as well as insights on how to successfully implement a high-throughput RNAi screen. |
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