| Chapter title |
High-Throughput RNAi Screening
|
|---|---|
| Chapter number | 11 |
| Book title |
High-Throughput RNAi Screening
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6337-9_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6335-5, 978-1-4939-6337-9
|
| Authors |
Yin, Hongwei, Kassner, Michelle, Hongwei Yin, Michelle Kassner |
| Editors |
David O. Azorsa, Shilpi Arora |
| Abstract |
High-throughput RNA interference (HT-RNAi) is a powerful tool that can be used to knock down gene expression in order to identify novel genes and pathways involved in many cellular processes. It is a systematic, yet unbiased, approach to identify essential or synthetic lethal genes that promote cell survival in diseased cells as well as genes that confer resistance or sensitivity to drug treatment. This information serves as a foundation for enhancing current treatments for cancer and other diseases by identifying new drug targets, uncovering potential combination therapies, and helping clinicians match patients with the most effective treatment based on genetic information. Here, we describe the method of performing an in vitro HT-RNAi screen using chemically synthesized siRNA. |
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