Chapter title |
Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening.
|
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Chapter number | 8 |
Book title |
High-Throughput RNAi Screening
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6337-9_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6335-5, 978-1-4939-6337-9
|
Authors |
Hanshuo Zhang, Juan Li |
Editors |
David O. Azorsa, Shilpi Arora |
Abstract |
RNAi has now become a valuable research tool for cell-based high-throughput screening. However, traditional RNAi high-throughput methods are based on multi-well plates, relying on expensive instruments and complicated operations. In this chapter, we describe a method termed self-assembled cell microarray (SAMcell), which integrates micro-fabrication, reverse transfection, and RNAi technologies and allows for cell behavior investigations to be performed directly on the cell chip. This method has been successfully employed to perform large-scale functional screening assays to identify gene modulators of cell migration, cell proliferation, and cellular apoptosis. |
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