Chapter title |
Intravital Microscopy Analysis of Hepatic T Cell Dynamics
|
---|---|
Chapter number | 4 |
Book title |
T-Cell Differentiation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6548-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6546-5, 978-1-4939-6548-9
|
Authors |
Alexandre Pierre Benechet, Lucia Ganzer, Matteo Iannacone |
Editors |
Enrico Lugli |
Abstract |
T cells play critical roles in controlling hepatotropic viral infections and liver tumors. The protective capacity of these cells is mediated by antigen-experienced effector cells and depends on their ability to migrate to and traffic within the liver, recognize pathogen- or tumor-derived antigens, get activated and deploy effector functions.While some of the rules that characterize T cell behavior in the healthy and cancerous antigen-expressing liver have been characterized at the population level, we have only limited knowledge of the precise dynamics of T cell interactions with different kinds of liver cells at the single-cell level. Here, we describe in detail an intravital microscopy technique that allows the analysis of T cell dynamic behavior in the liver of anesthetized mice at high spatial and temporal resolution. A detailed understanding of the spatiotemporal dynamics of T cells within the liver is important for the rational design of targeted immunotherapeutic approaches for chronic liver infections and tumors. |
Mendeley readers
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Unknown | 14 | 100% |
Demographic breakdown
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Researcher | 3 | 21% |
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Student > Bachelor | 1 | 7% |
Other | 1 | 7% |
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Unknown | 2 | 14% |