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Mendeley readers
| Chapter title |
Strategies for T Helper Cell Subset Differentiation from Naïve Precursors
|
|---|---|
| Chapter number | 11 |
| Book title |
T-Cell Differentiation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6548-9_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6546-5, 978-1-4939-6548-9
|
| Authors |
Francesco Annunziato, Laura Maggi |
| Editors |
Enrico Lugli |
| Abstract |
In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specific polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 25% |
| Researcher | 3 | 19% |
| Student > Doctoral Student | 2 | 13% |
| Student > Master | 2 | 13% |
| Other | 2 | 13% |
| Other | 0 | 0% |
| Unknown | 3 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 6 | 38% |
| Medicine and Dentistry | 3 | 19% |
| Agricultural and Biological Sciences | 1 | 6% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Chemistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 25% |