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T-Cell Differentiation

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Cover of 'T-Cell Differentiation'

Table of Contents

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    Book Overview
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    Chapter 1 The Vast Universe of T Cell Diversity: Subsets of Memory Cells and Their Differentiation
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    Chapter 2 Basic Aspects of T Helper Cell Differentiation
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    Chapter 3 FACS Analysis of Memory T Lymphocytes
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    Chapter 4 Intravital Microscopy Analysis of Hepatic T Cell Dynamics
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    Chapter 5 Analysis of T Cell Activation by Confocal Microscopy
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    Chapter 6 Phenotypic and Functional Analysis of Antigen-Specific T Cell Exhaustion
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    Chapter 7 pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel
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    Chapter 8 Differentiation of Diverse Progenies of Memory T Cells from Naïve CD8+ T Cell Precursors
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    Chapter 9 Gammaretroviral Production and T Cell Transduction to Genetically Retarget Primary T Cells Against Cancer
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    Chapter 10 Measuring Telomerase Activity in Senescent Human T Cells Upon Genetic Modification
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    Chapter 11 Strategies for T Helper Cell Subset Differentiation from Naïve Precursors
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    Chapter 12 Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells
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    Chapter 13 Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation
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    Chapter 14 T-Cell Differentiation
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    Chapter 15 T-Cell Differentiation
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    Chapter 16 Single-Cell RNA Sequencing of Human T Cells
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    Chapter 17 Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry
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    Chapter 18 Developmental and Functional Assays to Study Murine and Human γδ T Cells
Attention for Chapter 7: pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel
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Chapter title
pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel
Chapter number 7
Book title
T-Cell Differentiation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6548-9_7
Pubmed ID
Book ISBNs
978-1-4939-6546-5, 978-1-4939-6548-9
Authors

Daisy Philips, Marlous van den Braber, Ton N. Schumacher, Pia Kvistborg

Editors

Enrico Lugli

Abstract

The development of peptide loaded major histocompatibility complexes (MHC) conjugated to fluorochromes by Davis and colleagues 20 years ago provided a highly useful tool to identify and characterize antigen-specific T cells. In this chapter we describe a multiplexing strategy that allows detection of high numbers of T cell responses in parallel.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 22%
Student > Postgraduate 1 11%
Professor 1 11%
Other 1 11%
Student > Master 1 11%
Other 1 11%
Unknown 2 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 33%
Immunology and Microbiology 3 33%
Medicine and Dentistry 1 11%
Unknown 2 22%