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Cohesin and Condensin

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Cover of 'Cohesin and Condensin'

Table of Contents

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    Book Overview
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    Chapter 1 A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts.
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    Chapter 2 An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex.
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    Chapter 3 Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl.
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    Chapter 4 Detection of Cohesin SUMOylation In Vivo.
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    Chapter 5 Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans.
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    Chapter 6 Protein and Chromosome Analysis in Mammalian Meiocytes.
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    Chapter 7 Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex.
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    Chapter 8 Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq.
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    Chapter 9 A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.
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    Chapter 10 Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing.
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    Chapter 11 Zebrafish as a Model to Study Cohesin and Cohesinopathies.
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    Chapter 12 Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase.
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    Chapter 13 Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins.
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    Chapter 14 The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair.
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    Chapter 15 A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells.
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    Chapter 16 ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells.
Attention for Chapter 2: An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex.
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Chapter title
An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex.
Chapter number 2
Book title
Cohesin and Condensin
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6545-8_2
Pubmed ID
Book ISBNs
978-1-4939-6543-4, 978-1-4939-6545-8
Authors

Yasuto Murayama, Frank Uhlmann

Editors

Kyoko Yokomori, Katsuhiko Shirahige

Abstract

The cohesin complex is involved in a broad range of chromosomal biology, including DNA repair, gene transcription as well as sister chromatid cohesion. Cohesin is a large, ring-shaped protein complex and is thought to entrap DNA molecules inside of its ring. The unique DNA association is central to cohesin function and requires its ATPase and another heterodimer complex called the cohesin loader. Here we describe the biochemical reconstitution of topological cohesin loading onto DNA using the purified fission yeast cohesin proteins.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 33%
Student > Ph. D. Student 2 22%
Professor 1 11%
Student > Bachelor 1 11%
Student > Master 1 11%
Other 0 0%
Unknown 1 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 44%
Biochemistry, Genetics and Molecular Biology 3 33%
Physics and Astronomy 1 11%
Unknown 1 11%