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Cohesin and Condensin

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Cover of 'Cohesin and Condensin'

Table of Contents

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    Book Overview
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    Chapter 1 A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts.
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    Chapter 2 An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex.
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    Chapter 3 Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl.
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    Chapter 4 Detection of Cohesin SUMOylation In Vivo.
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    Chapter 5 Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans.
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    Chapter 6 Protein and Chromosome Analysis in Mammalian Meiocytes.
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    Chapter 7 Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex.
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    Chapter 8 Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq.
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    Chapter 9 A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.
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    Chapter 10 Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing.
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    Chapter 11 Zebrafish as a Model to Study Cohesin and Cohesinopathies.
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    Chapter 12 Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase.
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    Chapter 13 Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins.
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    Chapter 14 The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair.
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    Chapter 15 A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells.
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    Chapter 16 ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells.
Attention for Chapter 12: Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase.
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Chapter title
Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase.
Chapter number 12
Book title
Cohesin and Condensin
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6545-8_12
Pubmed ID
Book ISBNs
978-1-4939-6543-4, 978-1-4939-6545-8
Authors

Preksha Gupta, Thais Lavagnolli, Hegias Mira-Bontenbal, Matthias Merkenschlager, Gupta, Preksha, Lavagnolli, Thais, Mira-Bontenbal, Hegias, Merkenschlager, Matthias

Editors

Kyoko Yokomori, Katsuhiko Shirahige

Abstract

Cohesin is essential for the maintenance of chromosomes through the cell cycle. In addition, cohesin contributes to the regulation of gene expression and the organization of chromatin in interphase cells. To study cohesin's role in gene expression and chromatin organization, it is necessary to avoid secondary effects due to disruption of vital cohesin functions in the cell cycle. Here we describe experimental approaches to achieve this and the methods applied to define cohesin's role in interphase.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Professor 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 50%
Agricultural and Biological Sciences 2 50%