| Chapter title |
The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair.
|
|---|---|
| Chapter number | 14 |
| Book title |
Cohesin and Condensin
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6545-8_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6543-4, 978-1-4939-6545-8
|
| Authors |
Xiangduo Kong, Alexander R. Ball Jr., Kyoko Yokomori, Alexander R. BallJr., Alexander R. Ball |
| Editors |
Kyoko Yokomori, Katsuhiko Shirahige |
| Abstract |
In addition to their mitotic and transcriptional functions, cohesin plays critical roles in DNA damage response (DDR) and repair. Specifically, cohesin promotes homologous recombination (HR) repair of DNA double-strand breaks (DSBs), which is conserved from yeast to humans, and is a critical effector of ATM/ATR DDR kinase-mediated checkpoint control in mammalian cells. Optical laser microirradiation has been instrumental in revealing the damage site-specific functions of cohesin and, more recently, uncovering the unique role of cohesin-SA2, one of the two cohesin complexes uniquely present in higher eukaryotes, in DNA repair in human cells. In this review, we briefly describe what we know about cohesin function and regulation in response to DNA damage, and discuss the optimized laser microirradiation conditions used to analyze cohesin responses to DNA damage in vivo. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 38% |
| Student > Ph. D. Student | 2 | 25% |
| Professor | 1 | 13% |
| Student > Master | 1 | 13% |
| Unknown | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 50% |
| Agricultural and Biological Sciences | 2 | 25% |
| Unknown | 2 | 25% |