Chapter title |
Characterizing F-actin Disassembly Induced by the Semaphorin-Signaling Component MICAL.
|
---|---|
Chapter number | 8 |
Book title |
Semaphorin Signaling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6448-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6446-8, 978-1-4939-6448-2
|
Authors |
Jimok Yoon, Ruei-Jiun Hung, Jonathan R. Terman |
Editors |
Jonathan R. Terman |
Abstract |
The MICALs are a family of phylogenetically conserved cytoplasmic proteins that modulate numerous cellular behaviors and play critical roles in semaphorin-plexin signaling. Our recent results have revealed that the MICALs are an unusual family of actin regulatory proteins that use actin filaments (F-actin) as a direct substrate-controlling F-actin dynamics via stereospecific oxidation of conserved methionine (Met44 and Met47) residues within actin. In particular, the MICALs have a highly conserved flavoprotein monooxygenase (redox) enzymatic domain in their N-terminus that directly oxidizes and destabilizes F-actin. Here, we describe methods to characterize MICAL-mediated F-actin disassembly using in vitro assays with purified proteins. |
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